MicroCT scans of an iodine stained prenatal rabbit Project ID: 0000C1184 Public

3 Media · 1 Specimen · Managed by: Rachel Racicot, Irina Ruf

Rachel Racicot and Irina Ruf The datasets herein represent a pilot study involving iodine staining and subsequent µCT scanning of a prenatal lagomorph. The specimen used is <i>Oryctolagus cuniculus</i> (SNG 17032), 57.8 mm long (from dorsal part of head to tail as measured from the µCT scans), collected in 1908, and was preserved in formalin. We used a preprepared 1% solution of Lugol’s iodine. This solution stained the specimen over the course of almost three months, and was exchanged every two weeks, or whenever the staining solution became clear rather than dark brown. The amount of penetration visible in the µCT scans of the whole specimen after 5 weeks and 2 days extends to a few millimeters into the specimen. After 7 weeks more penetration is visible, extending almost fully to the interior of the specimen except for parts of the heart, liver, and other deep structures in this area. After 11 weeks and 2 days, only a small part near the posterior liver and nearby area is not fully penetrated by the stain. The contrast between tissues is sufficient to see the majority of the internal anatomy after 11 weeks.